Metabolomics Facility

metabolomics-machine-2

Instrumentation

The following instruments are housed and maintained in the CRI Metabolomics Facility for use by CRI investigators and collaborators:

Basic metabolite analysis:

  • NOVA BioProfile 4 (measures glucose, lactate, glutamine and glutamate from tissue culture medium).
  • Hansatech Oxygraph (measures oxygen consumption in intact cells or isolated mitochondria).

High performance liquid chromatography and ultra-high performance liquid chromatography:

  • Hitachi L-8900 Amino Acid Analyzer (clinical-grade analyzer to quantify free amino acids in plasma, culture medium, or tissue extracts).
  • Hitachi Ultra-High Performance Liquid Chromatography system (rapid analysis of amino acids and other metabolites).

Gas chromatography/mass spectrometry:

  • GC/MS#1: Agilent 7890 networked to Agilent 5973 Mass Selective Detector
  • GC/MS#2: Agilent 7890 networked to Agilent 5973 Mass Selective Detector

Triple quadrupole mass spectrometry:

  • AB SCIEX 5500

The following instruments are available in other departments within UT Southwestern: