Mouse Genome Engineering Facility

Mouse Genome Engineering Facility

Getting Started

Users of the facility need to submit a completed service request form to Yu Zhang.

Once you have submitted your request with an IDR, please arrange to drop off your DNA or other materials to NL12.120F. Procedures will be done in the order that requests are received.

In general, three separate injection days (about 500 embryos) will be injected with DNA or Cas9, and all viable blastocysts will be implanted into pseudopregnant females. If there are technical failures of some of these injections, we will perform additional injections. It is possible that after these injections, the project will not yield successful mice. In these cases, a new project will need to be initiated.

Service Costs

CRI users:

DNA/RNA injection

$2,800

ES cell injection

$2,000

Sperm cryopreservation

$200

Rederivation (IVF or embryos)

$1,700

ICSI

$2,000

UTSW users:

DNA/RNA injection

$3,500

ES cell injection

$2,500

Sperm cryopreservation

$300

Rederivation (IVF or embryos)

$2,100

ICSI

$2,500