Sequencing Facility

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Library Prep

Workflow overview:

  1. Prepare samples (RNA, ChIP DNA, genomic DNA, etc.)
  2. Choose a library preparation kit.
  3. Library prep.
  4. Library QC.
  5. Library pooling (if using multiplexed library). See this guide for pooling strategies using the Illumina TruSeq library prep kit.
  6. Choose a library preparation kit. Library prep kits can be obtained from several vendors:

Once you have decided which kit to use for library prep, please follow the protocols provided by the manufacturer.

Pay attention to the minimum amount of DNA or RNA required for each kit, and whether the provided indexing (or barcoded adaptor) system is compatible with Illumina sequencing technology.

Please contact Jian Xu if you have any questions.