The Moody Foundation Flow Cytometry Facility
The Moody Foundation Flow Cytometry Facility at CRI is open to all scientists of the Children’s Research Institute and UT Southwestern Medical Center. Facility staff will operate the instruments for any untrained users during work hours (9 a.m. to 6 p.m.). Individuals are not allowed to operate the instruments without proper training and completion of the facility training course provided by one of the Flow Facility staff members for the instrument they wish to operate.
Successful use of flow cytometry technology requires mastery of sample preparation, instrumentation
and analysis software. Flow cytometers are relative measurement devices. Everything is dependent upon proper set up and use of the instrument. Misuse of the instrument will inadvertently lead to mistakes in data acquisition and/or analysis that will result in incorrect, misleading, or unreliable/irreproducible results. Proper training in the use of flow cytometry will help you avoid these problems.
The Facility is open to qualified self-operators 24 hours a day, 365 days per year.
The course, offered by the Flow Facility staff to operate the FACSCanto RUO or FACS LSRFortessa SORP, consists of two sessions. The first session will be a theoretical class lasting approximately two hours to familiarize you with Flow Cytometry. The second sessopn will be spent on the instrument itself where we discuss general laboratory procedures and how to QC, operate, and clean the instrument.
Multi-laser analysis and cell sorting require experience with a digital analyzer running FACSDiva (i.e., FACSCanto RUO, FACS LSRFortessa). A complimentary training session will be offered by the Flow Facility staff.