Sequencing Facility

About

CRI’s Sequencing Facility is a shared resource committed to enhancing research capacity for CRI and UT Southwestern scientists through the use of genomics-based technologies. The facility has an Illumina NextSeq 500 desktop Next Generation Sequencing (NGS) system optimized for high throughput, high speed and long reads.

Our core services include:

  • NextSeq500 sequencing
    • Midsized project with a handful of samples
    • Transcriptome mRNA-seq
    • Transcriptome total RNA-seq
    • ChIP-seq
    • Exome sequencing
    • Targeted sequencing
    • Amplicon sequencing (e.g. CRISPR screening libraries)
  • 10xGenomics scRNA-seq and scATAC-seq
  • Agilent Tapestation 2200 RNA/DNA analysis
  • Qubit DNA quantification
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Process

INSTRUMENTATION AND PRICING

The facility has an Illumina NextSeq 500 desktop Next Generation Sequencing (NGS) system optimized for high throughput, high speed and long reads.

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SAMPLE PREP AND SUBMISSION

Our Facility provide the detailed guideline for library preparation and DNA analysis service using Qubit (DNA concentration) and Tapestation (DNA fragment size).

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FACILITY ACCESS

The facility is open to all scientists at CRI, UTSW and other interested institutions. For access, please contact us below at least a week before you plan to run sequencing.

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By the Numbers

Projects Completed

388

Users

56

Our Team

Yoon

Yoon Jung Kim, Ph.D.

Core Research Scientist

Jian Xu, Ph.D.

Scientific Director of the Facility