CRI Sequencing Facility

About

CRI’s sequencing facility is a shared resource committed to enhancing research capacity for CRI and UT Southwestern scientists through the use of genomics-based technologies. The facility has an Illumina NextSeq 2000 and a MiSeq desktop Next Generation Sequencing (NGS) systems optimized for high throughput, high speed, and long reads.

Our core services include:

  • NextSeq 2000 sequencing platform
    • P1 to P3 flow cells for 100 million to 1.2 billion reads
    • Suitable for a variety of medium to high sample throughput projects, including all types of RNA-seq, ChIP-seq, Cut&Run, HiC, and single-cell sequencing, etc.
  • MiSeq sequencing platform
    • Nano, Micro, and Normal flow cells for 1 ~ 25 million reads.
    • Suitable for small libraries, including amplicon sequencing, targeted sequencing, and CRISPR screen sequencing
  • 10X Genomics instrument for scRNA-seq and scATAC-seq library preparation
  • Agilent Tapestation 4150 RNA/DNA analysis
  • Qubit DNA quantification
  • Covaris ME220 focused-ultrasonicator
Contact Us
sequencing facility

Process

SAMPLE PREP AND SUBMISSION

Our Facility provide the detailed guideline for library preparation and DNA analysis service using Qubit (DNA concentration) and Tapestation (DNA fragment size).

Learn More

FACILITY ACCESS

The facility is open to all scientists at CRI, UTSW and other interested institutions. For access, please contact us below at least a week before you plan to run sequencing.

Contact Us

PRICING

The facility has an Illumina NextSeq 2000 and a MiSeq desktop Next Generation Sequencing (NGS) system optimized for high throughput, high speed and long reads.

Learn More

By the Numbers

Projects Completed

697

Users

135

Our Team

Yoon, sequencing facility

Yoon Jung Kim, Ph.D.

Assistant Professor, Research

Sihan Wu, Ph.D.

Scientific Director of the Facility