The Moody Foundation Flow Cytometry Facility

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Instrumentation

The facility is equipped with  three cells sorters (two FACS Aria II SORP and one FACS Aria Fusion SORP, all in biosafety hood for sorting primary human cells) and two cells analyzers. One analyzer is a special order FACS LSRFortessa SORP with High Throughput Sampler (plate loader) that provides fully automated and rapid sample acquisition and an enhanced dual FSC detection system (PMT + diode) with the ability to detect 0.2um particles from noise. The other analyzer is a special order (12 parameters) FACS Canto RUO with tubes loader. These instruments operate on a PC-based platform using FACSDiva (version 8) software.

Analyzer

FACS Canto RUO

facs_canto_overview The FACSCanto RUO is equipped with three lasers (405nm, 488nm, and 633 nm) that offer simultaneous 12-parameter detection and a FACSLoader. This state-of-the-art instrument offers fixed optical alignment as well as automated compensation and housekeeping routines making it highly desirable to operate.

FACS LSRFortessa SORP

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The FACS LSRFortessa SORP is equipped with four lasers (405nm, 488nm, 561 and 633 nm) that offer simultaneous 18-parameters detection and a High Throughput Sampler (HTS) that provides fully automated and rapid sample acquisition. The system also includes an enhanced dual FSC detection system (PMT + diode) with the ability to detect 0.2µm particles from noise. This state-of-the-art instrument offers high power lasers that improve the detection of rare events as well as automated compensation and housekeeping routines making it highly desirable to operate.

Cells Sorter

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FACS Aria II SORP (4 lasers and 5 lasers) / FACS Aria Fusion SORP

The FACSAria II SORP/FACS Aria Fusion SORP offers state-of-the-art features in the most advanced cell sorter currently available on the market. The BD FACSAria II SORP/FACS Aria Fusion SORP has air-cooled lasers and fixed-alignment cuvette flow cell, which allows achieving superior fluorescence sensitivity. The BD FACSAria delivers high-speed multicolor sorting with digital acquisition rates of up to 20,000 events/second. It is able to achieve sort into two- and four-way bulk sorting devices for a variety of tube sizes, or can sort using Automated Cell Deposition Unit (ACDU) to multi-well plates or microscope slides. Two FACSAria II SORP are available in the facility:

One FACSAria Fusion SORP is available in the facility:

The three FACSAria (II and Fusion) have been placed in a class II biosafety cabinet designed to provide personnel, product, and environmental protection from potentially hazardous, aerosolized particulates.

Confocal Microscopy

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Leica TCS SP8

The Leica TCS SP8 has been designed for confocal microscopy with optimal photon efficiency and high speed. All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging. Backing up this sensitive detection are a high speed scanning system with up to 428 frames per second, large field of view of field number 22 and accelerated Z-stacking by a novel mode for the SuperZ galvanometer called Galvoflow. This suits the instrument for the most demanding samples, highest speed while offering full confocal resolution.