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Instrumentation

The Facility is equipped with a 2 BD FACSAria II SORP and 1 BD FACSCanto RUO Special Order. These instruments operate on a PC-based platform using FACSDiva software.

Analyzer

FACS Canto RUO Special Order

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The FACSCanto RUO Special Order is equipped with three lasers (405nm, 488nm, and 633 nm) that offer simultaneous 12-parameter detection and a FACSLoader. This state-of-the-art instrument offers fixed optical alignment as well as automated compensation and housekeeping routines making it highly desirable to operate.

Cells Sorter

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FACS Aria II SORP (4 lasers and 5 lasers)

The FACSAria II SORP offers state-of-the-art features in the most advanced cell sorter currently available on the market.

The FACSAria II SORP has air-cooled lasers and fixed-alignment cuvette flow cell, which allows achieving superior fluorescence sensitivity. BD FACSAria II delivers high-speed multicolor sorting with digital acquisition rates of up to 20,000 events/second. It is able to achieve sort into two- and four-way bulk sorting devices for a variety of tube sizes, or can sort using Automated Cell Deposition Unit (ACDU) to multi-well plates or microscope slides.

Two FACSAria II SORP are available in the Facility:

  • Four-laser system (405nm, 488nm, 561nm, 633nm), which simultaneously collects multiple emission wavelengths (up to 13 fluorescents detectors)
  • Five-laser system (305nm, 405nm, 488nm, 561nm, 633nm) that accommodate up to 17 parameters -15 colors and 2 scatters

The two FACSAria II SORP have been placed in a class II biosafety cabinet designed to provide personnel, product, and environmental protection from potentially hazardous, aerosolized particulates.