Instrumentation
Our facility is equipped with three cell sorters and four cell analyzers. To schedule time with our instruments, please visit CRI PPMS.
Analyzers
FACS Canto RUO
The FACSCanto RUO is equipped with three lasers (405 nm, 488 nm and 633 nm) that offer simultaneous 12-parameter detection and a FACSLoader. This state-of-the-art instrument offers fixed optical alignment as well as automated compensation and housekeeping routines, making it highly desirable to operate.
FACS Lyric
The FACSLyric is equipped with three lasers (405 nm, 488 nm and 633 nm) that offer simultaneous 14-parameter detection and a FACS Universal Loader. This state-of-the-art instrument combines speed, simplicity and automation to help laboratories be more productive. It is designed to maximize the accuracy and reproducibility of data over time between instruments – whether at the same site or across the globe.
Two FACS LSRFortessa
The LSRFortessa are equipped with four lasers (405 nm, 488 nm, 561 nm and 633 nm) that offer simultaneous 18-parameter detection and a High Throughput Sampler (HTS) that provides fully automated and rapid sample acquisition.
On one of our system (LSRFortessa SORP) has also an enhanced dual FSC detection system (PMT + diode) with the ability to detect 0.2 µm particles from noise.
This state-of-the-art instrument offers high power lasers that improve the detection of rare events as well as automated compensation and housekeeping routines, making it highly desirable to operate.
Cells Sorters
FACS Aria II SORP (4 lasers and 5 lasers) / FACS Aria Fusion SOP / FACSymphony S6
The FACSAria II SORP, FACS Aria Fusion SORP, and FACSymphony S6 offer state-of-the-art features in the most advanced cell sorter on the market. BD FACSAria II SORP and FACS Aria Fusion SORP have air-cooled lasers and fixed-alignment cuvette flow cells, which allow achieving superior fluorescence sensitivity. BD FACSAria delivers high-speed multicolor sorting with digital acquisition rates of up to 20,000 events/second. It is able to achieve sort into two- and four-way bulk sorting devices for a variety of tube sizes and can sort using Automated Cell Deposition Unit (ACDU) to multiwell plates or microscope slides.
The FACSymphony adds cutting edge technology to the performance of the BD FACS Aria. The FACSymphony is configurable up to 50 parameters to define phenotypically distinct subpopulations and has the flexibility to sort them up to 6 ways or into plates or slides. Low-noise electronics and PMT detectors with high sensitivity at respective detector wavelengths are combined with optics designed to maximize signal collection of high quality data.
Quick reference guides are available for the FACSAria II SORP, FACSAria Fusion SORP, and FACSymphony S6. Please review our guidelines for help with sample preparation for sorting.
Two FACSAria II SORP are available in the facility.
Four-laser system (405 nm, 488 nm, 561 nm, 633 nm), which simultaneously collects multiple emission wavelengths (up to 13 fluorescents detectors): Aria II (4-lasers) Panel Worksheet
Five-laser system (305 nm, 405 nm, 488 nm, 561 nm, 633 nm) that accommodate up to 17 parameters – 15 colors and 2 scatters: Aria II (5-lasers) Panel Worksheet
Also available is one FACSAria Fusion SORP, which is a five-laser system (305 nm, 405 nm, 488 nm, 561 nm, 633 nm) that accommodate up to 20 parameters – 18 colors and 2 scatters: Aria Fusion (5-lasers) Panel Worksheet
In addition is one FACSymphony S6, which is also a five-laser system (355 nm, 405 nm, 488 nm, 561 nm, 637 nm) that accommodates up to 32 parameters – 30 colors and 2 scatters: FACSymphony S6 (5-lasers) Panel Worksheet
The three FACSAria (II and Fusion) as well as the FACSymphony S6 are in class II biosafety cabinets designed to provide personnel, product and environmental protection from potentially hazardous, aerosolized particulates.
Confocal Microscopy
The Leica TCS SP8 has been designed for confocal microscopy with optimal photon efficiency and high speed. All optical components are matched toward preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging. Backing up this sensitive detection are a high speed scanning system with up to 428 frames per second, large field of view of field number 22 and accelerated Z-stacking by a novel mode for the SuperZ galvanometer called Galvoflow. This suits the instrument for the most demanding samples, highest speed while offering full confocal resolution.
