Moody Foundation Flow Cytometry

Tools

We offer a number of solutions in our facility to help with experiments including design support, storage solution and analysis assistance.

FlowJo

The Flow Cytometry Facility administers the UTSW site license for FlowJo analysis software, which allows us to offer a heavily discounted version of the software for our users. To participate in this program, you must have a CRI PPMS account and be affiliated with UT Southwestern. To obtain a serial number for your computer, place the order through CRI PPMS.

Our flow cytometry experts are available to help analyze data but you can also find videos and tutorials with demo data on the FlowJo website.

Spectral Viewer

Several flow cytometry companies, including BD Biosciences eBioscience and ThermoFisher Scientific, have created fluorochrome spectral viewers to facilitate panel design and have a theoretical prediction of the compensation. These online tools are designed to aid customer understanding of important principles, dependencies, and relationships involved in selecting fluorochromes for multicolor flow cytometry. Users of these tools should ultimately rely on their own judgment when selecting fluorochromes for multicolor panels and experiments.

Panel Designer

One difficult task that people encounter when they start making multicolor flow cytometry panels is the process of finding antibodies and determining which fluorochromes to use for our instruments. The CRI Flow Cytometry Facility recommends using Fluorofinder to help you in this task.

Setting up a Fluorofinder account:

  1. Go to http://cri-utsw.fluorofinder.com
  2. “Create an account” if you want your panel to be saved
  3. Select the instrument you want to use, and follow the instructions
Cytobank

Cytobank provides a turnkey storage solution for your flow cytometry data. Cytobank allows researchers a new way to manage, search and analyze their FCS files over the web. The analysis system is designed to incorporate annotations and make it easier to create figures for lab meetings, publications or sharing. Cytobank also facilitates discussions about flow cytometry across and within groups. Users can share experiment data and details quickly and easily through the web. Collaborators can start with the result and drill down to the underlying single cell data.

A key challenge in flow cytometry is communicating the results along with sufficient experiment details to allow for additional analysis. In Cytobank, users provide these details during the course of analysis, allowing you to start with analyzed or gated data, drill down to the raw data and export gated or raw data in tab-delimited or FCS formats.

Protocols

Here are some examples of basics protocols:

  • Cell Cycling (.pdf)
  • Surface Staining (.pdf)
  • Intra Cellular Staining (ICS) (.pdf)
  • Sample preparation for sorting (.pdf)
  • SOPs “Biosafety while sorting” (.pdf)