The CRI Mouse Genome Engineering Core Facility was established in 2014 to produce transgenic and knockout mouse models. The facility provides low-cost DNA microinjection services, ES cell manipulation and microinjection services to all CRI and UT Southwestern investigators. We focus on using Cas9 mediated genome engineering methods to generate novel mouse models. The facility also provides services on strain rederivation, sperm cryopreservation and IVF procedures for resurrecting sperms.
Dr. Hao Zhu is the director of the facility with responsibility for its overall operation. Yu Zhang is the lead scientist in the facility and has over 10 years of mouse engineering experience.
Users of the facility need to submit a completed service request form to Yu Zhang.
- CRISPR Injection
- ES Cell Injection
- Intracytoplasmic Sperm Injection (ICSI)
- Sperm Freeze
- Transgenic Injection
Once you have submitted your request with an IDR, please arrange to drop off your DNA or other materials to NL12.120F. Procedures will occur in the order that we receive requests.
In general, three separate injection days (about 500 embryos) will be injected with DNA or Cas9, and all viable blastocysts will be implanted into pseudopregnant females. If there are technical failures of some of these injections, we will perform additional injections. It is possible that after these injections, the project will not yield successful mice. Such cases require initiation of a new project.
Intracytoplasmic morphologically selected sperm injection (IMSI) and intracytoplasmic sperm injection (ICSI) require a high level of skill and careful manipulation under a microscope. An important determining factor in the success of these procedures is the performance of the microscope and manipulator. We use the Olympus IX73 which delivers superior optical performance and an ideal working environment.