Sequencing Facility

Instrumentation

The Illumina NextSeq 500 is the desktop NGS system optimized for speed and long reads. Each cartridge (or flow cell) produces up to 400 million reads with read lengths between 75 bp and 150 bp by either single (SR) or paired end (PE) reads to generate up to 120 Gb of data and more than 75 percent of high-quality reads (Q30). Multiple samples can be multiplexed onto a single cartridge to more efficiently distribute the reads. The turnaround time can be as fast as 30 hours per cartridge.

The NextSeq 500 is ideal for:

  • Midsized project with a handful of samples
  • Transcriptome RNA-seq
  • ChIP-seq
  • Exome sequencing
  • Targeted sequencing
  • Amplicon sequencing
  • De novo sequencing

Available Sequencing Types:

  • 1 x 75bp (SR75)
  • 1 x 150bp (SR150)
  • 2 x 75bp (PE75)
  • 2 x 150bp (PE150)